AST Assay:Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory.
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [11]. ..
Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [ ]. ..
Diffusion-based Assay:Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory.
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [11]. ..
Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [ ]. ..
Concentration Assay:Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory.
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [11]. ..
Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [ ]. ..
Microscopy:Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory.
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [11]. ..
Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [ ]. ..
Imaging:Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory.
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [11]. ..
Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [ ]. ..
Bacteria:Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory.
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [11]. ..
Article Title: Comparative Assessment of Rapid Identification and Antimicrobial Susceptibility Testing Methods for Bloodstream Infections in a Non-24/7 Clinical Microbiology Laboratory
Article Snippet: .. Rapid antimicrobial susceptibility testing (AST) from positive blood cultures is conducted with the several following methods: (1) direct inoculation of positive blood culture broth onto Mueller–Hinton agar followed by disk diffusion testing (e.g., direct blood culture disk diffusion testing of Clinical and Laboratory Standards Institute [CLSI], rapid AST of European Committee on Antimicrobial Susceptibility Testing); (2) processing the broth to obtain a bacterial pellet for determining the minimum inhibitory concentration (MIC) using conventional automated AST systems (e.g., direct AST using BD Phoenix M50 [Becton-Dickinson and Company, Sparks, NV, USA]); (3) utilizing microscopy-based imaging systems to detect morphological changes in bacteria under antimicrobial exposure, thereby assessing bacterial growth in real-time (e.g., Accelerate Pheno system [Accelerate Diagnostics, Tucson, AZ, USA], QuantaMatrix dRAST [QuantaMatrix, Seoul, Republic of Korea]); and (4) molecular methods that detect antimicrobial resistance (AMR) genes using gene-specific primers directly from positive blood culture bottles (e.g., FilmArray BCID2 panel) [ ]. ..
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